|Data Set Name||SporeSat2 Ground Experiments – ZeroDay Results|
|Data Set Description||
This data set contains results from ground experiments using a lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD detects differential calcium ion concentrations outside of individual cells. Each experimental run is a run of a single bioCD containing 32 wells. Each well is loaded with a single live fern spore (sample) or an intentionally dead spore (negative control) or is left empty (negative control). Each experimental run is as ZeroDay# and this dataset contains ZeroDay4 through ZeroDay13. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.
Files are organized into:
|Level of Processing||Summarized - Digital|
|Data Files [Available online]|
|Data Collection Site(s)||Investigator laboratory|
|Data Set Review or Technical Comments||
File Descriptions: Compare Variables across Zero Day tests: Summary of Experimental Conditions for Each ZeroDay# Run
Cals: Every Zeroday run has a cals.txt file that contains the experimental calibration parameters for the 4 RTDs on the bioCD. The matlab analysis code should be pointed to the folder containing this file to read the data.
Event Timeline: Every Zeroday run has a Event_Timeline.txt file that contains the event timeline. The matlab analysis code should be pointed to the folder containing this file to read the data.
ZeroDayX Load Checks: Every Zeroday run has a ZeroDayX_Load_Checks.txt file that contains the well loading variable (live spore, dead spore, or empty) and the outcome of the well load check (good or bad). The matlab analysis code should be pointed to the folder containing this file to read the data.
Raw Data/science: Every ZeroDay run contains a raw data folder that include log files from the run, the most important of which is the science.csv file which contains the voltage differential recordings from the calcium selective electrode pairs.
SporeSat Matlab Code 2015a/_pass.txt, SporeSat Matlab Code 2015a/shadedErrorBar.m, SporeSat Matlab Code 2015a/SporeSat_Data_Analysis_v0_5.m, SporeSat Matlab Code 2015a/SporeSat_Data_Analysis_v0_6.
SporeSat Matlab Code 2015a/tightfig.m: Together these 5 files contain the matlab code that runs a basic graphing and analysis of the the raw data files from a single ZeroDayX run. ZeroDayX_Results: The output from the Matlab analysis code for each ZeroDayX analysis. ZeroDay_4_6_7_8_DATA: Summarized and aggregated analyzed data from ZeroDay 4, 6, 7, and 8. ZeroDay_4_6_7_8_Statistical Testing: Summary of statistical methods and outcome for aggregated analyzed data from ZeroDay 4, 6, 7, and 8.
ZeroDay_4_6_7_8_summary: Summary of results for aggregated analyzed data from ZeroDay 4, 6, 7, and 8. Then pdf file of ZeroDay_4_6_7_8_summary.pptx.
ZeroDay 8_10_11_12zeroday_background: Analysis of Background noise from negative control wells for ZeroDay 8, 10, 11, and 12. Then pdf file of ZeroDay 8_10_11_12zeroday_background.
ZeroDay_8_10_11_12_background_noise: Analysis of Background noise from negative control wells for ZeroDay 8, 10, 11, and 12. Then pdf file of ZeroDay_8_10_11_12_background_noise.
|Measurement or Analysis Technique||
Treatment: Light induced germination during 24 hours of rotation (hypergravity) at controlled temperature.
|Responsible NASA Official||
The NASA representative responsible for providing this information is:
Johnson Space Center LSDA Office
Project Manager: Pamela A. Bieri