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EXPERIMENT INFORMATION

Growth Hormone Regulation, Synthesis, and Secretion in Microgravity: II. Immunohistochemical Analysis of Hypothalamic Hormones (COS 1887-23)
Principal Investigators
Research Area:
Endocrinology
Neurophysiology
Species Studied
Scientific Name: Rattus norvegicus Species: Rat Strain: Wistar

Description
Objectives:

It was originally anticipated that, for this experiment, blocks of hypothalamic tissue would be prepared for radioimmunoassay of hypophysiotropic hormones mediating somatic growth (growth hormone-releasing factor, somatostatin) and stress-related corticotropin secretion (corticotropin-releasing factor). Even within the hypothalamus, however, each is also rather broadly distributed in cell bodies and/or axons that bear no ostensible relationship to their hypophysiotropic functions. Because of this, it was decided to attempt to employ immunohistochemical methods to better localize effects of space flight on these neuropeptide systems.


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Publications
Sawchenko, P.E. et al.: The Distribution of Growth Hormone-Releasing Factor (GRF)-Immunoreactivity in the Central Nervous System of the Rat: An Immunohistochemical Study Using Antisera Directed Against Rat Hypothalamic GRF. Journal of Comparative Neurology, vol. 237, 1985, pp. 100-115.

Sawchenko, P.E.: Evidence for Differential Regulation of CRF and Vasopressin-Immunoreactivities in Parvocellular Neurosecretory and Autonomic-Related Projections of the Paraventricular Nucleus. Brain Research, vol. 437, 1987, pp. 253-263.

Vale, W. et al.: Growth Hormone Regulation, Synthesis, and Secretion in Microgravity: II. Immunohistochemical Analysis of Hypothalamic Hormones. Final Reports of the U.S. Experiments Flown on the Soviet Biosatellite Cosmos 1887. J.P. Connolly, R.E. Grindeland, and R.W. Ballard, eds., NASA TM-102254, 1990, pp. 459-463.

Keywords
Hormones
Hypothalamus
Somatostatin
Somatotropin-releasing hormone

Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Parameters
Content, Corticotropin-releasing factor, in hypothalamus, rat, Immunocytochemistry
Content, Growth hormone (GH), in hypothalamus, rat, Immunocytochemistry
Content, Growth hormone releasing factor (GRF), in hypothalamus, rat, Immunocytochemistry
Content, Somatostatin, in hypothalamus, rat, Immunocytochemistry
Content, Vasopressin, in hypothalamus, rat, Immunocytochemistry
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Cosmos 1887 09/29/1987 10/11/1987 13 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
C1887-24.2
Protocol / Approach
Hardware Items