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EXPERIMENT INFORMATION

Effect of Zero Gravity on Myosin Isoform Expression in Rodent Skeletal Muscle (COS 2044-25)
Principal Investigator
Research Area:
Muscle physiology
Species Studied
Scientific Name: Rattus norvegicus Species: Rat Strain: Wistar

Description
Objectives:

The purpose of the present study was to extend previous findings on the effects of space flight on the biochemical properties of rodent fast-twitch and slow-twitch knee extensor muscles. A primary focus was to ascertain the effect on whole homogenate protein, myofibril protein yields, myofibril ATPase, isomyosin distribution patterns, and alpha-glycerolphosphate dehydrogenase activity in vastus intermedius (VI), vastus lateralis (VL), and vastus medialis (VM) muscles.


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Publications
Baldwin, K.M. et al.: Effect of Zero Gravity on Myosin Isoform Expression in Rodent Skeletal Muscle. Final Reports of the U.S. Experiments Flown on the Soviet Biosatellite Cosmos 2044. Vol. 1. J.P. Connolly, R.E. Grindeland, and R.W. Ballard, eds., NASA TM-108802, 1994, pp. 341-356.

Thomason, D.B. et al.: Altered Actin and Myosin Expression in Muscle During Exposure to Microgravity. Journal of Applied Physiology, supl., vol. 73, no. 2, 1992, pp. S90-S93.

Keywords
Adenosinetriphosphatase (ATPase)
Contractile proteins
Glyceraldehydephosphate dehydrogenase
Muscle fibers, fast-twitch
Muscle fibers, slow-twitch
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Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Parameters
Activity, enzymic, Adenosine triphosphatase (ATPase), vastus complex, muscle, rat, Assay
Activity, enzymic, Alpha-glycerolphosphate dehydrogenase, vastus intermedius, muscle, rat, Homogenate assay (units)
Activity, enzymic, Alpha-glycerolphosphate dehydrogenase, vastus lateralis, muscle, rat, Homogenate assay (units)
Activity, enzymic, Alpha-glycerolphosphate dehydrogenase, vastus medialis, muscle, rat, Homogenate assay (units)
Content, Myofibril protein, in vastus lateralis, muscle, rat, Gel Electrophoresis
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Cosmos 2044 09/15/1989 09/29/1989 14 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
C2044-7
Protocol / Approach
Proposal Date
6/17/1988
Proposal Source
88-OSSA-9
Hardware Items