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Effect of Spaceflight on Microbial Gene Expression in Saccharomyces cerevisiae (Yeast GAP 1) (CGBA04CN 1_2)
Principal Investigator
Research Area:
Species Studied
Scientific Name: Saccharomyces cerevisiae Species: Yeast, brewer's
Study Type

The objective of this experiment is to identify the S. cerevisiae genes that provide a selective advantage or disadvantage for cell survival in the space environment. The genes which convey a survival advantage and those that determine a survival disadvantage in response to spaceflight will be identified by whole genome microarray-mediated fitness expression profiling of diploid homozygous and heterozygous S. cerevisiae cultures. The results will be compared to those obtained when the same experiments are performed, with the identical microbes cultured under ground-based conditions of modeled microgravity.

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Wilson JW, Ott CM, Quick L, Davis R, Höner zu Bentrup K, Crabbé A, Richter E, Sarker S, Barrila J, Porwollik S, Cheng P, McClelland M, Tsaprailis G, Radabaugh T, Hunt A, Shah M, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, CdeBaca A, Narayan S, Benjamin J, Goulart C, Rupert M, Catella L, Schurr MJ, Buchanan K, Morici L, McCracken J, Porter MD, Pierson DL, Smith SM, Mergeay M, Leys N, Stefanyshyn-Piper HM, Gorie D, Nickerson CA. Media ion composition controls regulatory and virulence response of Salmonella in spaceflight. PLoS ONE. 2008;3(12):e3923.

Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, Pierson DL. Microbial responses to microgravity and other low-shear environments. Microbiol Mol Biol Rev. 2004 Jun;68(2):345-61.

Wilson JW, Ott CM, Hoener zu Bentrup K, Ramamurthy R, Quick L, Porwollik S, Cheng P, McClelland M, Tsaprailise G, Radabaugh T, Hunt A, Fernandez D, Richter E, Shah M, Kilcoyne M, Joshi L, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, Goulart C, Rupert M, Stodieck L, Stafford P, Catella L, Schurr MJ, Buchanan Morici L, McCracken J, Allen P, Baker-Coleman C, Hammond T, Vogel J, Nelson R, Pierson DL, Stefanyshyn-Piper HM, Nickerson CA. "Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq." Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16299-304.

Johanson K, Allen PL, Gonzales-Villalobos RA, Nesbit J, Nickerson CA, Höner zu Bentrup K, Wilson JQ, Ramamurthy R, D'Elia R, Muse KE, Hammond J, Freeman J, Stodieck LS, Hammond TG. Haploid deletion strains of Saccharomyces cerevisiae that determine survival during spaceflight. Acta Astronautica, (2007) 640-471. [NTRS]

Crabbé A, Nielsen-Preiss SM, Woolley CM, Barrila J, Buchanan K, McCracken J, Inglis DO, Searles SC, Nelman-Gonzalez MA, Ott CM, Wilson JW, Pierson DL, Stefanyshyn-Piper HM, Hyman LE, Nickerson CA. Spaceflight enhances cell aggregation and random budding in Candida albicans. PLoS One. 2013 Dec 4;8(12):e80677.

Data Information
Data Availability
Archiving in progress. Data is not yet available for this experiment.

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Expedition 8 10/18/2003 04/29/2004 195 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Martha Del Alto
Institutional Support
National Aeronautics and Space Administration (NASA)
Protocol / Approach
Proposal Source
Hardware Items