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EXPERIMENT INFORMATION

Effect of Spaceflight on Microbial Gene Expression and Virulence (Microbe) (2001092)
Principal Investigator
Research Area:
Microbiology
Species Studied
Scientific Name: Pseudomonas aeruginosa Species: Bacterium

Description
OBJECTIVES:
Determine the effects of spaceflight, and nutritional status, on gene expression and virulence of three microbrial pathogens: Salmonella typhimurium, Pseudomonas aeruginosa, and Candida albicans.


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Publications
Wilson JW, Ott CM, Quick L, Davis R, Höner zu Bentrup K, Crabbé A, Richter E, Sarker S, Barrila J, Porwollik S, Cheng P, McClelland M, Tsaprailis G, Radabaugh T, Hunt A, Shah M, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, CdeBaca A, Narayan S, Benjamin J, Goulart C, Rupert M, Catella L, Schurr MJ, Buchanan K, Morici L, McCracken J, Porter MD, Pierson DL, Smith SM, Mergeay M, Leys N, Stefanyshyn-Piper HM, Gorie D, Nickerson CA. Media ion composition controls regulatory and virulence response of Salmonella in spaceflight. PLoS ONE. 2008;3(12):e3923. pubmed.gov

Wilson JW, Ott CM, Hoener zu Bentrup K, Ramamurthy R, Quick L, Porwollik S, Cheng P, McClelland M, Tsaprailise G, Radabaugh T, Hunt A, Fernandez D, Richter E, Shah M, Kilcoyne M, Joshi L, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, Goulart C, Rupert M, Stodieck L, Stafford P, Catella L, Schurr MJ, Buchanan Morici L, McCracken J, Allen P, Baker-Coleman C, Hammond T, Vogel J, Nelson R, Pierson DL, Stefanyshyn-Piper HM, Nickerson CA. "Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq." Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16299-304. pubmed.gov

Crabbé A, Schurr MJ, Monsieurs P, Morici L, Schurr J, Wilson JW, Ott CM, Tsaprailis G, Pierson DL, Stefanyshyn-Piper H, Nickerson CA. Transcriptional and proteomic response of Pseudomonas aeruginosa PAO1 to spaceflight conditions involves Hfq regulation and reveals a role for oxygen. Appl Environ Microbiol. 2011 Feb;77(4):1221-30. pubmed.gov

Crabbe, A., Pyke, B., Monsierus, P., Van Houdt, R., Nickerson, C.A., Leys, N., and P. Cornelis. Response of Pseudomonas aeruginosa to low shear modeled microgravity involves AlgU regulation. Environ. Microbiol. 2010 Jun;12(6):1545-64. pubmed.gov

Crabbé A, Nielsen-Preiss SM, Woolley CM, Barrila J, Buchanan K, McCracken J, Inglis DO, Searles SC, Nelman-Gonzalez MA, Ott CM, Wilson JW, Pierson DL, Stefanyshyn-Piper HM, Hyman LE, Nickerson CA. Spaceflight enhances cell aggregation and random budding in Candida albicans. PLoS One. 2013 Dec 4;8(12):e80677. pubmed.gov

Sarker S, Ott CM, Barrila J, Nickerson CA. Discovery of Spaceflight-regulated Virulence Mechanisms in Salmonella and other Microbial Pathogens: Novel Approaches to Commercial Vaccine Development. Gravitational and Space Biology, 2010 Sep;23(2):75-78.

Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
STS-115 09/09/2006 09/21/2006 12 days

Additional Information
Other Key People
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
Microbe
Protocol / Approach
Synchronous control
Proposal Source
ILSRA-2001