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EXPERIMENT INFORMATION

Novel Explorations Into the Interactions Between Light and Gravity Sensing in Plants - Seedling Growth-3 (ARC00XX145)
Principal Investigators
Research Area:
Plant biology
Species Studied
Scientific Name: Arabidopsis thaliana Species: Mouse-ear cress

Description
OBJECTIVES:

The major goals of this project are to determine how gravity and light responses influence each other in plants and to better understand the cellular signaling mechanisms involved in plant tropisms. This proposed project builds on our previous spaceflight experiments on the ISS (TROPI-1, 2006; TROPI-2, 2010) with the EMCS using the model plant Arabidopsis. In this new proposed project, we plan to confirm and extend our discovery during the recent TROPI-2 ISS experiment of a novel red-light-based positive phototropic response in plant shoots.

The combined experiments will be divided into four flight payloads in order to maximize the science return from the original proposals. The experiments are to be carried out with 3+1 flights in series. The first three flight experiments are designed to accomplish specific aims enumerated in the two NASA and ESA approved proposals. The last flight experiment is intended to allow a final flight to confirm and/or extend the findings from the first three.

Thus, the specific objectives of this proposed research are: (1) To confirm and characterize the novel red light-dependent phototropic response in flowering plants. (2) To investigate the relationship between light and gravity by measuring thresholds in fractional gravity. (3) To determine whether the red light effect on blue-light-based phototropism is a direct or indirect effect.

Seedling Growth 1: Phototropism
Seedling Growth 2: Tropisms, Auxin, and Cell Culture
Seedling Growth 3: Proliferating Cell Structure
Seedling Growth 4: Synthesis/Experiment Contingency

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Publications
Vandenbrink JP, Herranz R, Medina FJ, Edelmann RE, Kiss JZ. A novel blue-light phototropic response is revealed in roots of Arabidopsis thaliana in microgravity. Planta. Dec. 2016, vol. 244, no. 6, pp. 1201-1215.pubmed.gov

Vandenbrink, J.P.; Kiss, J.Z.; Herranz, R.; and Medina, F.J.: Light and gravity signals synergize in modulating plant development. Frontiers in Plant Science, vol. 5, 2014, p. 563. [DOI]

Vandenbrink, J.P. and Kiss, J.Z. (2016). Spaceflight Procedures and Operations Utilized during the seedling growth experiments. Gravitational and Space Research, 4(2), 47-57.

Crabbé A, Schurr MJ, Monsieurs P, Morici L, Schurr J, Wilson JW, Ott CM, Tsaprailis G, Pierson DL, Stefanyshyn-Piper H, Nickerson CA. Transcriptional and proteomic response of Pseudomonas aeruginosa PAO1 to spaceflight conditions involves Hfq regulation and reveals a role for oxygen. Appl Environ Microbiol. 2011 Feb;77(4):1221-30. pubmed.gov

Valbuena MA, Manzano A, Vandenbrink JP, Pereda-Loth V, Carnero-Diaz E, Edelmann RE, Kiss JZ, Herranz R, Medina FJ. The combined effects of real or simulated microgravity and red-light photoactivation on plant root meristematic cells. Planta. 2018 Jun 8. pubmed.gov [DOI]

pubmed.gov

Vandenbrink JP, Kiss JZ. Preparation of a spaceflight experiment to study tropisms in Arabidopsis seedlings on the International Space Station Methods Mol Biol. 2019;1924:207-14. pubmed.gov [DOI]

Herranz R, Vandenbrink JP, Villacampa A, Manzano A, Poehlman WL, Feltus FA, Kiss JZ, Medina FJ. RNAseq analysis of the response of Arabidopsis thaliana to fractional gravity under blue-light stimulation during spaceflight. Front Plant Sci. 2019 Nov 26;10:1529. [DOI]

Kiss JZ, Wolverton C, Wyatt SE, Hasenstein KH, van Loon JJWA. Comparison of microgravity analogs to spaceflight in studies of plant growth and development. Front Plant Sci. 2019 Dec 6;10:1577. [DOI]

Manzano A, Villacampa A, Sáez-Vásquez J, Kiss JZ, Medina FJ, Herranz R. The importance of Earth reference controls in spaceflight-omics research: Characterization of nucleolin mutants from the Seedling Growth experiments. iScience. 2020 Nov 20;23(11):101686. pubmed.gov [DOI]

Manzano A, Villacampa A, Sáez-Vásquez J, Kiss JZ, Medina FJ, Herranz R. The importance of Earth reference controls in spaceflight-omics research: Characterization of nucleolin mutants from the Seedling Growth experiments. iScience. 2020 Nov 20;23(11):101686. pubmed.gov [DOI]

Vandenbrink JP, Herranz R, Poehlman WL, Alex Feltus F, Villacampa A, Ciska M, Javier Medina F, Kiss JZ. RNA-seq analyses of Arabidopsis thaliana seedlings after exposure to blue-light phototropic stimuli in microgravity. Am J Bot. 2019 Nov 10. pubmed.gov [DOI]

Villacampa A, Ciska M, Manzano A, Vandenbrink JP, Kiss JZ, Herranz R, Medina FJ. From spaceflight to Mars g-levels: Adaptive response of A. thaliana seedlings in a reduced gravity environment is enhanced by red-light photostimulation. Int J Mol Sci. 2021 Jan 18;22(2):899. pubmed.gov [DOI]

Data Information
Data Availability
Archiving in progress. Data is not yet available for this experiment.

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
SpaceX_6 04/14/2015 05/21/2015 37 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Sylvain Costes
Institutional Support
National Aeronautics and Space Administration (NASA)
Proposal Date
Proposal Source
NNH09ZTT002N