Effect of Deep Space Radiation on Human Hematopoietic Stem and Progenitor Cell Function (NCC958IIH00404)
Cell and molecular biology
Scientific Name: Homo sapiens Species: Human
Scientific Name: Mus musculus Species: Mouse
The original aims of this project were as follows: 1) Investigate the cellular consequences of exposing human hematopoietic stem (HSC) and progenitor (HPC) cells to an environment which simulates the radiation environment of deep space, 2) Examine the molecular consequences of exposing human hematopoietic stem cells to an environment which simulates the radiation environment of deep space, and 3) Design potential countermeasures to obviate or negate cellular and molecular damage discerned during the course of carrying out Aims 1 and 2.
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The first step was to develop a radiation exposure model that is more relevant to the types of radiation flight crews will be exposed to during longer duration lunar and Mars missions. In order to do so, low dose, multi-dose, and mixed particle exposure of normal human hematopoietic cells to H and Fe was carried out. H and FE were chosen because they are the main constituents of galactic cosmic rays and solar particle events. The functional consequences of such exposure on progenitor and stem cell function in vitro and in xenograft studies employing NOD-SCID mice were tested. Effects on DNA repair pathways were studied. The effects of shielding, as well as free radical scavengers were also examined.
It was found that very low doses of either particle are tolerated but that mixed particles are much more damaging. Further, shielding and radio-protectants appear to be able to ameliorate damage. In summary, much has been done to characterize the damage of various types, combinations, and doses of deep space radiations on normal human hematopoietic progenitor cells. This information is valuable for estimating risk of catastrophic bone marrow failure and the utility of specific countermeasures for mitigating such damage. Some of this information may be useful for estimating risk of development of hematologic malignancies post-mission.
Dose-response relationship, radiation
Hematopoietic stem cells
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Linear energy transfer (LET)
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Human hematopoietic cells
Human Research Program (HRP) Human Research Roadmap (HRR) Information
Crew health and performance is critical to successful human exploration beyond low Earth orbit.
The Human Research Program (HRP) investigates and mitigates the highest risks to human health
and performance, providing essential countermeasures and technologies for human space exploration.
Risks include physiological and performance effects from hazards such as radiation, altered gravity,
and hostile environments, as well as unique challenges in medical support, human factors,
and behavioral health support. The HRP utilizes an Integrated Research Plan (IRP) to identify
the approach and research activities planned to address these risks, which are assigned to specific
Elements within the program. The Human Research Roadmap is the web-based tool for communicating the IRP content.
The Human Research Roadmap is located at: https://humanresearchroadmap.nasa.gov/
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for information of how this experiment is contributing to the HRP's path for risk reduction.
Managing NASA Center
National Space Biomedical Research Institute (NSBRI)
Responsible NASA Representative
National Aeronautics and Space Administration (NASA)
2003 Biomedical Research & Countermeasures 03-OBPR-04
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