Investigators will compare cfDNA and cRNA profile among samples collected on the earth and during space flight. The results should provide key information including (i) low-frequency gene mutation events in internal tissue cells, (ii) cellular origin of cfDNA which represents sites of cellular damage or excessive tissue turnover by alteration of stem cell activity or cell death, and (iii) cRNA expression profile to screen gene-specific biomarker of stress response. Direct sequencing of cfDNA by ultra-high-throughput sequencing could detect mutations in DNA. DNA mutation rate will be a reliable indicator of genetic damage by space radiation in the human body under normal DNA repair function. Investigators will also perform detection and quantification of epigenetic modification (methylation) of cfDNA. Since methylation pattern of DNA is specific for cell types, this information will be used to identify the cellular origin of cfDNA. cRNA expression profile indicates cellular pathways that respond against environmental stress. If there are any genes specifically up or down-regulated during flight, these genes could be important to enhance environmental tolerance. Such genes may be targets for drug development or specific biomarker candidates.
Results for this investigation are unavailable. This is an international experiment. NASA does not currently have an agreement with international space partners to archive their data in the LSDA.
|Mission||Launch/Start Date||Landing/End Date||Duration|
|Expedition 54||12/14/2017||02/27/2018||75 days|
|Expedition 55||02/27/2018||06/03/2018||96 days|
|Expedition 56||06/03/2018||10/04/2018||123 days|
|Expedition 57||10/04/2018||12/20/2018||77 days|
|Expedition 58||12/18/2018||03/14/2019||85 days|
|Expedition 59||03/14/2019||06/24/2019||102 days|