To study induction and repair of clustered DNA damages, the investigators irradiated HT1080 cells stably expressing green-fluorescent protein tagged XRCC1 (EGFP-XRCC1) with Fe, Si and Oxygen particles and immunostained with 53BP1 (double strand breaks) and OGG1 (base damage). Using these as surrogate markers, they noticed differential induction of clustered DNA damages by different HZE particles. Moreover, the number of cells harboring the un-repaired clustered damages was higher in Fe irradiated cells, as compared to Si and O.
To study the differential induction of different types of DNA damages, including DSBs, SSB and base damages, along the ion tracks traversed by HZE particles, the investigators have established stable cells lines expressing RFP-XRCC1 and YFP-53BP1 and confirmed their recruitment to the sites of DNA lesions generated by Fe, Si particles and gamma-rays. Using this approach, they directly monitored induction of single and DSBs in single living cells.
To study the genomic instability caused by charged particles, The investigators irradiated HT1080 cells with Fe particles (1Gy, 1GeV/n) and examined the chromosomal aberrations 24 hours after irradiation. When they analyzed the metaphase spreads, they noticed different types of chromosomal abnormalities in Iron irradiated cells as compared with mock-irradiated cells.
To determine the cell cycle delay induced by charged particles, the investigators irradiated exponentially growing HT1080 cells with Fe particles and collected samples before (pre-IR) and 0.5, 1, 2, 4, 8, 12, 24 and 48 hours after irradiation and checked for cell cycle delay by Flow cytometer. Results showed that irradiation of cells with Fe ions causes extended period of G2/M arrest.
No datasets exist for this study. A final report was archived.
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