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Mechanisms of the Repair of HZE Induced DNA Double-Strand Breaks in Human Cells (NNX07AU42G)
Principal Investigator
Research Area:
Radiation health
Species Studied
Scientific Name: Species: Cells, human

DNA repair capability is one of the key indicators reflecting the biological consequences of cellular exposure to genotoxic insults. Determining the biochemical nature of the DNA damages induced by HZE particles and identifying the factors that are required for the repairing of these DNA lesions will provide tools to assess individual radiation susceptibility and also to validate risk assessment for human exposure to HZE particles. The goals of this study were two fold: 1) Develop new approaches to determine the types and spatial relationship among different DNA lesions formed along the dense ionizing tracks induced by HZE particles in human cells and to dissect the cellular capability to repair these DNA lesions in situ; and 2) Identify molecular pathways or factors that may be able to help the human cell respond efficiently to HZE particle-induced DNA lesions. For this purpose, investigators tested their hypothesis that specific nucleases, Artemis and/or Werner Syndrome protein (WRN), contribute specifically to the repair of complex DNA lesions induced by HZE particles. Investigators also aimed to determine the role of homologous recombination factors in repair of HZE particle-induced double strand breaks (DSBs). With these new approaches on hand, the investigators sought to provide a semi-quantitative measurement for the generation and spatial distribution of clustered DNA lesions along the dense track induced by HZE particles and determine the relative contribution of these Nonhomologous end-joining and homologous end joining factors in the repair of DNA DSBs, but also able to identify whether these repair activities play any role in managing clustered DNA lesions generated by HZE.

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HZE particles
Radiation effects

Data Information
Data Availability
Archive is complete. Data sets are not publicly available but can be requested.
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Cell cycle
DNA damage
DNA lesions
DNA repair

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Ground 05/01/2009 In Progress

Human Research Program (HRP) Human Research Roadmap (HRR) Information
Crew health and performance is critical to successful human exploration beyond low Earth orbit. The Human Research Program (HRP) investigates and mitigates the highest risks to human health and performance, providing essential countermeasures and technologies for human space exploration. Risks include physiological and performance effects from hazards such as radiation, altered gravity, and hostile environments, as well as unique challenges in medical support, human factors, and behavioral health support. The HRP utilizes an Integrated Research Plan (IRP) to identify the approach and research activities planned to address these risks, which are assigned to specific Elements within the program. The Human Research Roadmap is the web-based tool for communicating the IRP content.

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Additional Information
Managing NASA Center
Johnson Space Center (JSC)
Responsible NASA Representative
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
Proposal Date
Proposal Source
2006 Radiation Biology NNJ06ZSA001N