1. To ensure that the air quality is microbiologically safe for crewmembers and to ensure compliance with existing acceptability limits established for microbial air sampling.
2. To ensure a microbiologically safe environment for crewmembers and to ensure compliance with existing acceptability limits established for microbial surface sampling.
3. To check for the presence of microbial contaminants in the potable water provided for crew use on the ISS and verify compliance with established water quality requirements.
Bacteria from surface and air samples are isolated on Tryptic Soy Agar for identification. Bacteria were isolated from water samples on the ISS using a filtration unit, the Microbial Capture Device (MCD) which contains an R3A medium. Bacteria are also isolated from water samples returned from the ISS to JSC in an archive water bag. Samples are processed using an R2A medium. Identification of bacteria was performed using either a VITEK Identification System (bioMérieux) or 16S genetic analysis.
The JSC Microbiology Laboratory has several isolates collected and identified during the microbial monitoring efforts available for research evaluation. Several types of microorganisms have been identified multiple times during sampling and are available for evaluation as a time course indicated as early, mid, and late in the life of the ISS. It is important to note that microbial identifications were performed during ground processing of samples returned from space flight. Further characterization was not performed. A partial list of microbial isolates collected can be downloaded for review and requested for further research.
|Mission||Launch/Start Date||Landing/End Date||Duration|
|Expedition 13||03/22/2006||09/24/2006||186 days|
|Expedition 19||03/26/2009||10/11/2009||199 days|
|Expedition 20||05/27/2009||10/11/2009||137 days|
|Expedition 21||10/11/2009||12/01/2009||51 days|
|Expedition 22||11/30/2009||03/18/2010||109 days|
|Expedition 23||03/18/2010||06/01/2010||75 days|
|Expedition 24||06/01/2010||09/25/2010||117 days|
|Expedition 25||09/24/2010||11/25/2010||31 days|
|Expedition 26||11/26/2010||03/16/2011||111 days|
|Expedition 27||03/14/2011||05/23/2011||70 days|
|Expedition 28||05/23/2011||09/15/2011||115 days|
|Expedition 29||09/16/2011||11/21/2011||40 days|
|Expedition 30||11/14/2011||04/27/2012||166 days|
|Expedition 31||04/27/2012||07/01/2012||65 days|
|Expedition 32||07/01/2012||09/16/2012||78 days|
|Expedition 33||09/16/2012||11/18/2012||63 days|
|Expedition 34||11/18/2012||03/15/2013||117 days|
|Expedition 35||03/15/2013||05/13/2013||58 days|
|Expedition 36||05/13/2013||09/10/2013||166 days|
|Expedition 37||09/10/2013||11/10/2013||61 days|
|Expedition 38||11/10/2013||03/10/2014||120 days|
|Expedition 39||03/10/2014||05/13/2014||64 days|
|Expedition 40||05/13/2014||09/10/2014||133 days|
|Expedition 41||09/10/2014||11/09/2014||29 days|
|Expedition 42||11/10/2014||03/11/2015||121 days|
|Expedition 43||03/11/2015||06/10/2015||91 days|
|Expedition 45||09/11/2015||12/11/2015||91 days|