The Aims of this project will be to collect mouse liver, bone marrow, thymus, and adrenal glands (as part of the kidney). Our group is interested in the impact of space flight on the B cell response to tetanus toxoid antigen immunization. Thymus and adrenals will be used for the assessment of stress on the animals. In addition, space flight also impacts lymphocyte responses in an organ-specific manner. Therefore, we propose to collect liver and bone marrow to make comparative analyses of IgH gene usage to the already planned spleen analysis.
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These tissues will be used to accomplish the following two specific aims:
1. Specific Aim 1: Collect thymus to weigh the tissue to compare relative thymic atrophy among treatment groups and adrenal glands to assess catecholamines. These tissues will be used to assess short- and long-term stress to the flight animals.
2. Specific Aim 2: Collect liver and bone marrow. These tissues will be used for the preparation of RNA to allow for a comparative analysis of B cell IgH usage between lymphoid compartments.
This experiment is currently in progress. Results will be available at the conclusion of the study.