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EXPERIMENT INFORMATION

Temporal Transcriptomics Analysis of Archived Spaceflight Biological Samples from ALSDA from 1991 to 2016 (ARC00XX204)
Principal Investigator
Species Studied
Scientific Name: Rattus norvegicus Species: Rat Strain: Sprague-Dawley

Description
OBJECTIVE:
The purpose of this study is to assess the quality of spaceflight tissues stored in Ames Life Science Data Archive (ALSDA) freezers. Garnering information – for downstream functional analysis such as generation of omics datasets – from tissues is, in part, dependent on the state of sample preservation. To assess the viability of a select group of tissues, RNA integrity number (RIN) values were calculated for RNA extracted from rodent livers. Rat livers from Space Launch System 1 (SLS-1) and mouse livers from Commercial Biomedical Test Module 3 (CBTM-3), Rodent Research 1 (RR1), and Rodent Research 3 (RR3) were tested. It was found that mean RIN values from CBTM-3, RR1, and RR3 were suitable for downstream functional analysis (RIN > 5) while the mean RIN value for SLS-1 was not (RIN = 2.5 ± 0.1). Information from this study could lay the foundation for future efforts in determining the types of assays that are most appropriate for different tissues in ALSDA freezers, which would maximize the scientific return on rare spaceflight samples.


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Keywords
RNA
Tissue preservation

Parameters
RNA Integrity Number (RIN)

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
PMA

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Proposal Date
Proposal Source
Unsolicited