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EXPERIMENT INFORMATION

Growth Hormone Regulation, Synthesis and Secretion in Microgravity: II. Hypothalamic GH-Releasing Factor, Somatostatin Immunoreactivity, and mRNA Levels (COS 2044-22)
Principal Investigator
Research Area:
Endocrinology
Species Studied
Scientific Name: Rattus norvegicus Species: Rat Strain: Wistar

Description
Objectives:

Immunohistochemical analysis from a previous investigation (Cosmos 1887) suggested preferential effects on hypophysiotropic principles involved in the regulation of growth hormone secretion and synthesis. To provide an additional, more penetrating analysis, this study attempted to complement immunohistochemical analysis of growth hormone-releasing factor (GRF) and somatostatin (SS) staining with quantitative, in situ assessments of messenger RNAs encoding the precursors for both these hormones.


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Publications
Sawchenko, P.E. et al.: Effects of Spaceflight on Hypothalamic Peptide Systems Controlling Pituitary Growth Hormone Dynamics. Journal of Applied Physiology, supl., vol. 73, no. 2, 1992, pp. S158-S165.

Sawchenko, P.E. et al.: Growth Hormone Regulation, Synthesis and Secretion in Microgravity: II. Hypothalamic GH-Releasing Factor, Somatostatin Immunoreactivity, and mRNA Levels in Microgravity. Final Reports of the U.S. Experiments Flown on the Soviet Biosatellite Cosmos 2044. Vol. 2. J.P. Connolly, R.E. Grindeland, and R.W. Ballard, eds., NASA TM-108802, 1994, pp. 173-182.

Keywords
Hypothalamus
Immunohistochemistry
Protein precursors
RNA, messenger (mRNA)
Somatotropin
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Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Parameters
Content, Corticotropin-releasing factor, hypothalamus, rat, Immunohistochemistry
Content, cRNA, hypothalamus, rat
Content, DNA, hypothalamus, rat
Content, Growth hormone releasing factor (GRF), in hypothalamus, rat, Immunocytochemistry
Content, mRNA, hypothalamus, rat, In situ hybridization, labeled antisense probes
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Cosmos 2044 09/15/1989 09/29/1989 14 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
C2044-27.2
Protocol / Approach
Proposal Date
6/17/1988
Proposal Source
88-OSSA-9
Hardware Items