Thirty (30ml) of blood was collected by venipuncture into heparinized tubes, mixed with HBSS, centrifuged, washed and resuspended in cultural medium. Isolated PBMC was separated into control, hydrocortisone, and DHEA groups to investigate involvement of the endocrine system in the expected immune alterations. Secretion of type 1 and 2 cytokines was measured to study cell-mediated and humoral immunities. Expression of cytokine mRNA was determined in order to study mechanisms of immune alterations. Distribution of cytokine secreting T-cells was analyzed by flow cytometry after cultivation. Distribution of T-cell subsets, NK cells and monocytes in whole blood was assessed by flow cytometry. Five (5) ml of urine was collected on the same schedule as blood samples (L-10 days preflight, and R+0, R+3 and R+15 days postflight).
Results are not available.