This experiment is in progress. However, preliminary results are available.
This project is comprised of two different experiments: (1) the identification of core dimensions of neurobehavioral responses to sleep loss (Specific Aim 1) using a historical database of cognitive, subjective, and physiological responses to acute and chronic sleep loss in healthy adults (N=640); and (2) using the core dimensions of neurobehavioral responses to sleep loss to prospectively assess the validity of biomarkers of susceptibility to the neurobehavioral deficits from sleep loss. Investigators are currently creating the historical database to achieve Specific Aim 1 by locating and formatting historical data from N=640 healthy adults. The historical database includes data from experiments manipulating sleep duration in healthy adults through both total sleep deprivation (TSD) and chronic partial sleep deprivation (PSD) protocols conducted over the past 25 years. Of the proposed total N=640 subjects, a subset of N=175 unique subjects participated in one of ten TSD experiments and a subset of N=465 unique subjects participated in one of nine PSD experiments. Data on ten primary outcomes collected across the 19 studies are being included in the statistical determination of the core dimensions of responses to sleep loss. In addition to the progress constructing the historical database, data collection on predictor biomarkers (Specific Aim 2) has begun.
During the second year of funding, assessments of predictor markers were collected in three currently funded scientific experimental protocols, which expose participants to total and/or chronic partial sleep deprivation. The five predictor markers are: (1) Resting arterial spin labeling (ASL fMRI) cerebral blood flow without sleep deprivation; (2) Resting psychomotor vigilance test (PVT) time on task (TOT) without sleep deprivation; (3) Heart rate variability (HRV) during PVT TOT; (4) Salivary a-amylase activity (sAA) during sleep deprivation; (5) Variations in genes regulating sleep, circadian, and cognitive functions.
Collected data includes neuroimaging of non-sleep deprived subjects at baseline, Psychomotor Vigilance Task (PVT) performance, electrocardiograms during the 20-minute PVT, saliva samples during PSD, and blood draws during both TSD and PSD. During year 2, data on predictor marker 1 have been collected on a total of N=18 unique TSD subjects and N=25 unique PSD subjects. Projected data collection on biomarker 1 for year 2 is expected to total of N=22 unique TSD and N=29 PSD subjects. Data on predictor markers, 2, 3, 4, and 5 have been collected on a total of N=23 unique TSD subjects and N=31 unique PSD subjects. Projected data collection on biomarkers 2, 3, 4, and 5 during year 2 is expected to total of N=27 unique TSD and N=35 PSD subjects. Data collection on the five biomarkers of Specific Aim 2 will continue in year 3 with a projected total of N=34 unique TSD subjects and N=34 unique PSD subjects. Additionally, data from N=24 unique subjects were collected in both TSD and PSD conditions.
|Mission||Launch/Start Date||Landing/End Date||Duration|