Skip to page content Mission Information

EXPERIMENT INFORMATION

Countermeasures Against Adverse Effects of Space Radiation (80NSSC19K0435)
Research Area:
Radiation biology
Species Studied
Scientific Name: Mus musculus Species: Mouse Strain: C57BL/6

Description
OBJECTIVES:
This is the first task book for Phase 1 study of our project entitled “Countermeasures against adverse effects of space radiation.” The primary goal of our Phase 1 study is to test the efficacy of apigenin (AP) for the prevention and mitigation of cancer in male mice due to exposure to space radiation as measured by incidence, aggressiveness, burden, and latency. Our main hypothesis is that AP exhibits its beneficial effects by suppression of radiation-induced inflammation and oxidative stress during the initiation/promotion steps of carcinogenesis. Although the emphasis will be on the mitigation of lung cancer and lymphoma/leukemia, other types of cancer will be recorded.

In this project, we will give food containing AP to male mice before and after exposure to heavy silicon (28Si) ions. There are four groups of mice. These are sham controls (Group 1, no AP diet, no radiation), mice receiving 0.5 Gy of 260 MeV/n 28Si ions radiation only and without AP diet (Group 2), mice receiving AP only with no radiation exposure (Group 3), and mice receiving both AP and radiation (Group 4). Groups of mice from each treatment will be used for a serial sacrifice schedule at 1 week and 6 months post-irradiation. This will test the ability of AP to counteract heavy-ion-induced early- and late-occurring inflammation and oxidative damage in various tissues linked to cancer that are the focus of our study (i.e., bone marrow, lung, thymus, spleen). All remaining mice will be observed for morbidity and mortality until they reach about 600-700 days of age. Our data enable the evaluation of countermeasure efficacy of AP across tissues at risk for cancer induction, i.e., bone marrow and the lung. This multi-tissue of the same exposed individual approach has not been used in space research.

Aim 1: Measure the levels of early- and late-occurring impairment of the immune system in multiple tissues (plasma, BM, spleen, thymus, lung, brain, and heart) of the same mouse (male and female) included in the serial sacrifice, with or without exposure to a single beam of 260 MeV/n 28Si ions and a mixed beam of protons 1 GeV protons and 260 MeV/n 28Si ions, with or without AP treatment.

Aim 2: Quantify the levels of early- and late-occurring oxidative damage in multiple tissues (plasma, BM, spleen, thymus, lung, brain, and heart) of the same mouse (male and female) included in the serial sacrifice, with or without exposure to a single beam of 260 MeV/n 28Si ions and a mixed beam of 1 GeV protons and 260 MeV/n 28Si ions, with or without AP treatment.

Aim 3: Determine the effectiveness of AP in prevention, mitigation or delay of lung cancer or lymphoma/leukemia in exposed male or female C57BL/6 mice after exposure to a single beam of 260 MeV/n 28Si ions or a mixed beam of protons 1 GeV protons and 260 MeV/n 28Si ions as determined by an incidence, aggressiveness, overall burden, and latency.


++ -- View more

Data Information
Data Availability
Archiving in progress. Data is not yet available for this experiment.

Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Ground 05/01/2009 In Progress

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Martha Del Alto
Institutional Support
National Aeronautics and Space Administration (NASA)
Proposal Date
01/31/2019
Proposal Source
2017 HERO 80JSC017N0001-BPBA