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EXPERIMENT INFORMATION

Plant Embryos and Fidelity of Cell Division in Space (9305010 1_2)
Principal Investigator
Research Area:
Cell and molecular biology
Developmental biology
Plant biology
Species Studied
Scientific Name: Hemerocallis Species: Daylily Strain: Autumn blaze

Description
OBJECTIVES:

This experiment was designed to test whether the cell division changes observed in daylily (Hemerocallis cv Autumn Blaze) embryos during previous space flight experiments result directly from microgravity or indirectly through factors associated with water availability and uptake. Preliminary results from STS-29, STS-47 and STS-65 showed that genetic abnormalities occur in these plants during space flight. Because ground-based studies indicated that hydration conditions can impact the integrity of chromosomes, it is possible that the results observed on these flights were not due to direct effects on the plants, but rather to indirect effects mediated by water availability to plant cells.


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Publications
Krikorian AD, Brennan J. Ancymidol and daylilies. The Daylily Journal. 1995;50(4):414-7.

Krikorian AD, Kann RP, Smith DL. Somatic embryogenesis in daylily (Hemerocallis). In: Bajaj YPS, ed. Biotechnology in Agriculture and Forestry. Springer-Verlag, Berlin, Heidelberg; 1995;31:285-93.

Krikorian AD. Hormones in tissue culture and micropropagation. In: Plant Hormones: Physiology, Biochemistry and Molecular Biology. Dordrecht, Netherlands: 1995;774-96.

Krikorian AD. Regeneration of plants from protoplasts of Hemerocallis (Daylily). In: Biotechnology in Agriculture and Forestry. Springer-Verlag, Berlin, Heidelberg; 1995;34:70-8.

Krikorian AD. Space Biology: Studies at Orbital Stations. Quarterly Review of Biology. 1995;70:513-4.

Krikorian AD. Space stress and genome shock in developing plant cells. ASGSB Bulletin 1995;9(1):63.

Krikorian AD. Strategies for "minimal growth maintenance" of cell cultures: a perspective on management for extended duration experimentation in the microgravity environment of a Space station. Bot Rev. 1996;62(1):41-108. [pubmed.gov]

Krikorian AD. Space Biology: Studies at Orbital Stations. By G.S. Nechitailo and A.L. Mashinsky; Mir Publishers, Moscow: 1993.

Keywords
Cell division
Chromosomes
Cells, cultured
Protoplasts

Data Information
Data Availability
Archiving in progress. Data is not yet available for this experiment.

Parameters
Chromosome breakage
Chromosome damage
Chromosome damage, bridges
Chromosome damage, deletions
Chromosome partitioning
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
STS-70 07/13/1995 07/22/1995 9 days

Additional Information
Managing NASA Center
Kennedy Space Center (KSC)
Responsible NASA Representative
Kennedy Space Center LSDA Level 3
Project Manager: Martha Del Alto
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
BRIC
Proposal Date
2/1/94
Proposal Source
NRA-93-OLMSA-05