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EXPERIMENT INFORMATION

Responses of Crown Gall Tissue to the Space Environment: Isozyme Patterns (COS 782-2)
Principal Investigator
Research Area:
Plant biology
Species Studied
Scientific Name: Daucus carota Species: Carrot

Description
Objectives:

Clinostats have been used to simulate conditions of zero gravity. Carrot crown gall tumors were reportedly larger with respect to both fresh and dry weights when grown under gravity-compensated (horizontally rotated) conditions on a clinostat. It has also been shown that such tumors display a peroxidase zymogram which differs from that of vertically rotated or stationary controls. This experiment compares peroxidase zymograms between tumor tissue grown in space and that grown on land-based clinostats.


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Publications
Hanchey, P. et al.: Isozyme Patterns in Gravity-Compensated Crown Gall Tissues. Phytopathology, vol. 65, 1975, pp. 1136-1138.

Hanchey, P.: Responses of Crown Gall Tissue to the Space Environment: Isozyme Patterns. Final Reports of U.S. Experiments Flown on the Soviet Satellite Cosmos 782. S.N. Rosenzweig and K.A. Souza, eds., NASA TM-78525, 1978, pp. 64-70.

Wells, T.R. and R. Baker: Gravity Compensation and Crown Gall Development. Nature, vol. 223, 1969,
pp. 734-735.

Keywords
Agrobacterium tumefaciens
Centrifugation
Gravitation
Isoenzymes
Peroxidases
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Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Parameters
Activity, enzymic, Acid phosphatase, carrot gall tumor, Electrophoresis, densitometry
Activity, enzymic, Acid phosphatase, carrot tissue, Electrophoresis, densitometry
Activity, enzymic, Esterase, acid phosphatase, peroxidase isoenzymes, carrot gall tumor, Electrophoresis, densitometry
Activity, enzymic, Esterase, acid phosphatase, peroxidase isoenzymes, carrot gall tumor, Electrophoresis, densitometry
Activity, enzymic, Esterase, carrot gall tumor, Electrophoresis, densitometry
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Cosmos 782 11/25/1975 12/15/1975 20 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
C782-8.4
Protocol / Approach