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EXPERIMENT INFORMATION

Actin mRNA and Cytochrome-C mRNA Concentrations in the Triceps Brachia Muscle of Rats (COS 1887-31)
Principal Investigator
Research Area:
Muscle physiology
Species Studied
Scientific Name: Rattus norvegicus Species: Rat Strain: Wistar

Description
Objectives:

Some skeletal muscles atrophy as a result of weightlessness and as a result of hind limb suspension. The content of protein is determined by the rate of protein synthesis and degradation. Any decrease in protein synthesis could be caused by decreases in mRNA concentrations. In suspended rat hind limbs, an increased protein degradation and a decreased protein synthesis were observed, as well as decreases in the concentration and content of alpha-actin mRNA and cytochrome-C mRNA. From these findings, it was hypothesized that the same pattern could be observed in the triceps brachia muscle of space-flown rats.


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Publications
Booth, F.W. et al.: Actin mRNA and Cytochrome-C mRNA Concentrations in the Triceps Brachia Muscle of Rats. Final Reports of the U.S. Experiments Flown on the Soviet Biosatellite Cosmos 1887. J.P. Connolly, R.E. Grindeland, and R.W. Ballard, eds., NASA TM-102254, 1990, pp. 275-278.

Morrison, P.R. et al.: Actin Synthesis Rate and mRNA Level Increase During Early Recovery of Atrophied Muscle. American Journal of Physiology, vol. 253, 1987, pp. C205-C209.

Morrison, P.R. et al.: Cytochrome c Protein-Synthesis Rates and mRNA Contents During Atrophy and Recovery in Skeletal Muscle. Biochemistry Journal, vol. 241, 1987, pp. 257-263.

Keywords
Muscular atrophy
Cytochrome c
Actins
RNA, messenger (mRNA)

Data Information
Data Availability
Archive is complete. No data sets are available for this experiment. Please Contact LSDA if you know of available data for this investigation.

Parameters
Content, Actin mRNA, in triceps, muscle, rat: RNA dot blots, autoradiography, laser beam densitometry, scintillation count
Content, Cytochrome, somatic, muscle, rat: RNA probe P32 hybridization
Content, RNA, in triceps, muscle, rat: RNA assay
Cytochrome-c: triceps
Weight, tissue, Triceps brachii, muscle, rat Balance (CGS units)
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Mission/Study Information
Mission Launch/Start Date Landing/End Date Duration
Cosmos 1887 09/29/1987 10/11/1987 13 days

Additional Information
Managing NASA Center
Ames Research Center (ARC)
Responsible NASA Representative
Ames Research Center LSDA Level 3
Project Manager: Helen Stewart
Institutional Support
National Aeronautics and Space Administration (NASA)
Alternate Experiment Name
C1887-12
Protocol / Approach
Hardware Items